Anti-Heparanase 1/HPSE Antibody Picoband™

Product Name

Anti-Heparanase 1/HPSE Antibody Picoband™

View all Heparanase/HPSE Antibodies

SKU/Catalog Number

PB9427

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Heparanase 1/HPSE Antibody Picoband™ catalog # PB9427. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Heparanase 1/HPSE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9427)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Heparanase 1, different from the related mouse and rat sequences by eight amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9427 is reactive to HPSE in Human, Rat

Applications

PB9427 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

61 kDa

Calculated molecular weight

61.149kDa

Background of Heparanase/HPSE

Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody (PB9427).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: Human Placenta Tissue Lysate at 50ug,
Lane 3: A549 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody (Catalog # PB9427) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 61 kDa. The expected band size for Heparanase 1 is at 61 kDa.

Gene/Protein Information For HPSE (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.2; Endo-glucoronidase; HEP; Heparanase; Heparanase-1; HPA1; HPAheparanase exon 9 and 10 deletion; HPR1; HPSE; HPSE1heparanase-1; HSE1; HSE1heparanase exon10-deletion HPSE HPA, HPA1, HPR11, HSE1, HPSE heparanase heparanase|endo-glucoronidase|heparanase-1

For more info on HPSE, check out the HPSE Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HPSE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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